Chloride Channel 5 antibody (Extracellular)

Catalog No. ABIN7886217

This Rabbit Polyclonal antibody specifically detects Chloride Channel 5 in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for Chloride Channel 5 antibody (Extracellular) (ABIN7886217)

Target: Chloride Channel 5 (CLCN5)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chloride Channel 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Chloride Channel 5 Antibody

Binding Specificity: AA 401-415, Extracellular

Purpose: A Rabbit Polyclonal Antibody to CLC-5 (CLCN5) Channel

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14,15 amino acid residues identical

Characteristics: Anti- Antibody (ABIN7043053 and ABIN7044124) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CDYENHFNTSKGGEL, corresponding to amino acid residues 401-415 of rat CLCN5 

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235090)

Blocking Peptide: (ABIN7235090)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Chloride Channel 5

Target: Chloride Channel 5 (CLCN5)

Alternative Name: CLCN5

Background:

Synonyms: CLCN5, Chloride channel 5, Chloride transporter ClC-5, H+/Cl- exchange transporter 5, DENTS

Description: CLC5 is a member of the voltage-dependent Cl- channel family that includes nine known members. Based on sequence similarity, CLC5 is closely linked to the CLC3 and CLC4 subunits. CLC-5 is mainly found in intracellular vesicles, particularly in early endosomes, where it is believed to contribute to their acidification.The channel is mainly expressed in the kidney, although it has also been found in the small intestine, brain and liver.Mutations in the CLC5 gene cause Dent's disease, an X-linked renal disorder.

Gene ID: 25749

NCBI Accession: NM_000084

UniProt: P51796