CNIH2, CNIH3 (AA 49-61), (Extracellular) antibody

Catalog No. ABIN7043072

The Rabbit Polyclonal anti-CNIH2, CNIH3 antibody is suitable to detect CNIH2, CNIH3 in samples from Rat. It has been validated for WB.

Quick Overview for CNIH2, CNIH3 (AA 49-61), (Extracellular) antibody (ABIN7043072)

Target: CNIH2, CNIH3

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Product Details

Binding Specificity: AA 49-61, Extracellular

Purpose: A Rabbit Polyclonal Antibody to CNIH2 and CNIH3

Specificity: Extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): This antibody will recognize both CNIH2 and CNIH3, but not the related proteins CNIH1 and CNIH4.

Predicted Reactivity: CNIH2 = Mouse,human identicalCNIH3 = Rat,mouse,human - 11,13 amino acid residues identical

Characteristics: Anti-CNIH2/3 (extracellular) Antibody (ABIN7043072, ABIN7044388 and ABIN7044389) is a highly specific antibody directed against a conserved sequence of the rat CNIH2 and CNIH3 proteins. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and thus is ideal for detecting the proteins in living cells. It has been designed to recognize CNIH2 and CNIH3 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: RARERLKNIERIC, corresponding to amino acid residues 49-61 of rat CNIH2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235145)

Blocking Peptide: (ABIN7235145)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details

Target: CNIH2, CNIH3

Background: Cornichon homolog 2, Cornichon homolog 3, Cornichon family AMPA receptor auxiliary protein 2, Cornichon family AMPA receptor auxiliary protein 3,Cornichon homologs (CNIHs) are a family of AMPA-type glutamate receptor (AMPAR) auxiliary subunits that modulate AMPAR ion channel function and trafficking. Cornichon 2 and 3 (CNIH2/3) are responsible for controlling the export of AMPARs from the endoplasmic reticulum and associate with synaptic AMPARs to modulate channel kinetics1,2.CNIHs are small proteins with three transmembrane domains that directly binds to AMPARs. Overexpression CNIHs and AMPA receptors causes their localization to the Golgi apparatus3.CNIH and TARP both compete for the binding to AMPAR subunits GluA2, GluA3, and GluA4 subunits suggesting both proteins bind to the same site. CNIH2/3 binding to AMPARs is dependent on AMPAR subunit composition and TARPs3,4.CNIH2/3 knock out mice shows alterations in synaptic content of GluA1-containing AMPARs, resulting in a reduction in AMPAR-mediated transmission and long-term potentiation2.

Alternative names: CNIH2/3, Cornichon homolog 2, Cornichon homolog 3, Cornichon family AMPA receptor auxiliary protein 2, Cornichon family AMPA receptor auxiliary protein 3

Gene ID: 361705, 690252

NCBI Accession: NM_182553

UniProt: Q5BJU5, D0Q0Y7