Cortactin antibody (Intracellular)

Catalog No. ABIN7043085

This anti-Cortactin antibody is a Rabbit Polyclonal antibody detecting Cortactin in WB and IHC. Suitable for Mouse.

Quick Overview for Cortactin antibody (Intracellular) (ABIN7043085)

Target: Cortactin (CTTN)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cortactin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Cortactin Antibody

Binding Specificity: AA 346-359, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Cortactin

Specificity: Intracellular.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-Cortactin Antibody (ABIN7043085, ABIN7045162 and ABIN7045163) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize Cortactin from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KTSNIRANFENLAK, corresponding to amino acid residues 346 - 359 of mouse Cortactin

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:1000

Comment:

Negative Control: (ABIN7235169)

Blocking Peptide: (ABIN7235169)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Cortactin

Target: Cortactin (CTTN)

Alternative Name: CTTN

Background: Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN,Cortical acting binding protein, Cortactin, is an actin-binding protein involved in cytoskeletal regulation1. Cortactin is a 63-65 kDa protein that consists of four major domains: N-terminal acidic (NTA), 6.5 tandems repeat, proline-rich domain, and the C-terminal Src homology 3 domains (SH3 domains)2 and it is regulated by post translational modifications such as phosphorylation and acetylation1.The actin filament network provides mechanical support as well as protrusive and contractile forces essential for directed cell motility and cell and tissue morphogenesis. Cortactin activates the Arp2/3 complex, the main regulator of actin polymerization, and thereby regulates the assembly of branched actin3.Overexpression of cortactin was shown to enhance cell migration, invasion, and tumor cell metastasis. It is also overexpressed in various types of cancer such as breast cancers as well as head and neck tumors1.

Alternative names: Src substrate cortactin, Oncogene EMS1, EMS1, Amplaxin, CTTN

Gene ID: 13043

NCBI Accession: NM_138565

UniProt: Q60598

Pathways: MAPK Signaling