DPYSL2 antibody (Intracellular)

Catalog No. ABIN7886523

The Rabbit Polyclonal anti-DPYSL2 antibody (ABIN7886523) specifically detects DPYSL2 in WB and IHC. The antibody is reactive with Rat samples.

Quick Overview for DPYSL2 antibody (Intracellular) (ABIN7886523)

Target: DPYSL2 (Dihydropyrimidinase-Like 2 (DPYSL2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DPYSL2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-DPYSL2 Antibody

Binding Specificity: AA 504-517, Intracellular

Purpose: A Rabbit Polyclonal Antibody to CRMP-2

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-CRMP2 Antibody (ABIN7043097, ABIN7044542 and ABIN7044543) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CEVSVTPKTVTPAS, corresponding to amino acid residues 504-517 of rat CRMP2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235204)

Blocking Peptide: (ABIN7235204)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for DPYSL2

Target: DPYSL2 (Dihydropyrimidinase-Like 2 (DPYSL2))

Alternative Name: DPYSL2

Background:

Synonyms: CRMP-2, Collapsin response mediator protein 2, Dihydropyrimidinase-related protein 2, DHPRP2, DRP-2, Dpysl2, Turned on after division 64 kDa protein, TOAD-64, Ulip2, Unc-33-like phosphoprotein 2

Description: Collapsin response mediator protein 2 (CRMP-2, DRP-2), is a kinesin-binding protein and a member of the CRMP family, a novel family of microtubule-associated proteins (MAPs). This family consists of 5 highly conserved phosphorylated proteins: CRMP1-CRMP5. CRMP-2 was originally identified in the regulation of microtubule dimerization. CRMP-2 is the first member of the CRMP family identified in primary neuronal cells1,2.Physiological functions of CRMP-2 protein include regulation of cell surface receptor endocytosis, kinesin-dependent axonal transport, growth cone collapse, polarity and differentiation of developing neurons and neurite outgrowth and the regulation of microtubule dynamics2.Studies suggest that CRMP-2 can modulate neuronal viability through the PI3K/mTOR/S6K pathway. In addition, CRMP-2 expression has been detected in non-neuronal cells such as leukocytes, fibroblasts and neuroblastoma1.Phosphorylation of CRMP-2 on specific Thr and Ser residues inactivates its activity, leading to inhibition of axonal growth and neuronal polarity. Knockdown of CRMP-2 shortens the total dendritic length and decreases dendritic branching3.

Gene ID: 25416

NCBI Accession: NM_001386

UniProt: P47942

Pathways: Regulation of Cell Size