FFAR1 antibody (3rd Extracellular Loop)

Catalog No. ABIN7886323

This Rabbit Polyclonal antibody specifically detects FFAR1 in WB, IF, IHC, FACS and LCI. It exhibits reactivity toward Rat.

Quick Overview for FFAR1 antibody (3rd Extracellular Loop) (ABIN7886323)

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FFAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-FFAR1 Antibody

Binding Specificity: 3rd Extracellular Loop, AA 244-259

Purpose: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 1 (GPR40)

Predicted Reactivity: 16 amino acid residues identical,Mouse - 15, human - 12

Characteristics: Anti-GPR40/FFAR1 (extracellular) Antibody (ABIN7043137, ABIN7044279 and ABIN7044280) is a highly specific antibody directed against an epitope of the rat Free fatty acid receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize FFAR1 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)NVASFINPDLEGSWRK corresponding to amino acid residues 244-259 of rat FFAR1

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IHC

Negative Control: (ABIN7235329)

Blocking Peptide: (ABIN7235329)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for FFAR1

Target: FFAR1 (Free Fatty Acid Receptor 1 (FFAR1))

Alternative Name: FFAR1

Background:

Synonyms: G-protein coupled receptor 40, Free fatty acid receptor 1

Description: Free fatty acids (FFAs) were for long believed to influence cellular metabolism. Hoever, the discovery that FFAs actually mediate their effects through one of three G-protein coupled receptors (GPCRs) - Free Fatty Acid Receptors 1-3 (FFAR1-3), has initiated a number of studies in order to assess their implication in health and disease1.Like all other GPCRs, FFAR1-3 have seven transmembrane domains, an extracellular N-terminal tail and an intracellular C-terminus.FFAR1 is activated by either medium or long, saturated or unsaturated fatty acids2,3, whereas FFAR2 and FFAR3 are activated by short chain fatty acids4,5. FFAR1 is coupled to Gq and leads to Ca2+ mobilization upon activation. FFAR2 couples to both pertussis-sensitive and insensitive G-proteins and also leads to increased intracellular Ca2+ levels. FFAR3 coupling to G-protein induces an increase in cAMP1.Distribution studies of all three FFA Receptors indicate that FFAR1 mRNA is quite elevated in the pancreas2,3. FFAR1 is also detected in the liver, heart, skeletal muscle, and brain, although this broad localization pattern is supported only by one study2. FFAR2 is expressed in many tissues like liver and colon6. FFAR3 is expressed in many tissues but shows highest expression in adipose tissue4. Relatively high expression of the receptor could be detected in pancreas, spleen, lymph nodes and bone marrow4,5.As mentioned above, the finding that FFAs' actions are mediated by GPCRs, prompted many studies in order to elucidate their roles in healthy and disease states. Such studies stipulate that FFAR1 may be involved in obesity and type 2 diabetes since circulating free fatty acid levels in the plasma are significantly elevated. On the other hand, FFAR2 and FFAR3 could be potential targets for treating irritable bowel disease and appetite control1.

Gene ID: 266607

NCBI Accession: NM_005303

UniProt: Q8K3T4

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Peptide Hormone Metabolism, Carbohydrate Homeostasis