FZD2 antibody (Extracellular)

Catalog No. ABIN7043141

Product Details anti-FZD2 Antibody

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Binding Specificity: AA 192-205, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Frizzled-2

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Rat

Predicted Reactivity: Human - identical, mouse - 13,14 amino acid residues identical

Characteristics: Anti-Frizzled-2 (FZD2) (extracellular) Antibody is directed against an epitope of rat Frizzled-2. Anti-Frizzled-2 (FZD2) (extracellular) Antibody (ABIN7043141, ABIN7044285 and ABIN7044286)) can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize FZD2 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GAPPRYATLEHPFH, corresponding to amino acid residues 192-205 of rat Frizzled-2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-FR022

Blocking Peptide: BLP-FR022

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for FZD2

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Alternative Name: FZD2 (FZD2 Products)

Background: Frizzled class receptor 2, Fz-2,Frizzled receptors (FZD) belong to the superfamily of G-protein coupled receptors (GPCR). They are classified to the frizzled subfamily for which 11 members have been identified to date, FZD1-10 and Smoothened1. They were first identified in Drosophila melanogaster.Like all GPCRs, Frizzled receptors have seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. However, these receptors lack many features of the conventional class A, B or C GPCRs2.Activation of these receptors by WNT, leads to numerous complex signaling pathways which are generally termed β-catenin-dependent and β-catenin-independent. It is noteworthy to mention that the β-catenin-dependent pathway involves the interaction of FZD with LRP6 or LRP5 co-receptors3.Frizzled receptors are ubiquitously expressed and play a crucial role in embryo development. They are also significantly involved in proliferation and differentiation. Indeed, dysregulation of FZD leads to various forms of cancer1. Scientific evidence also shows their involvement in axonal remodeling and neurotransmitter release1.They have been associated to Alzheimer's and Parkinson's diseases, depression, anxiety, hypoxia and ischemia. It is thus not surprising that they have become pharmacological targets in a number of diseases and disorders.

Alternative names: Frizzled-2 (FZD2), Frizzled class receptor 2, Fz-2

Gene ID: 64512

NCBI Accession: NM_001466

UniProt: Q08464

Pathways: WNT Signaling