Mesothelin antibody (C-Term, Intracellular)

Catalog No. ABIN7886314

The Rabbit Polyclonal anti-Mesothelin antibody is suitable to detect Mesothelin in samples from Rat. It has been validated for WB, IHC and IF.

Quick Overview for Mesothelin antibody (C-Term, Intracellular) (ABIN7886314)

Target: Mesothelin (MSLN)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Mesothelin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Mesothelin Antibody

Binding Specificity: AA 329-342, C-Term, Intracellular

Purpose: A Rabbit Polyclonal Antibody to G-Protein Coupled Estrogen Receptor 1

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - 13,14, human - 12,14 amino acid residues identical

Characteristics: Anti-GPER (GPR30) Antibody (ABIN7043144, ABIN7044267 and ABIN7044268) is a highly specific antibody directed against an epitope of rat G-protein coupled estrogen receptor 1. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPER from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ETFRDKLRLYVAQK, corresponding to amino acid residues 329-342 of rat GPER

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235353)

Blocking Peptide: (ABIN7235353)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Mesothelin

Target: Mesothelin (MSLN)

Alternative Name: GPER1

Background:

Synonyms: G-protein coupled estrogen receptor 1, GPER1, GPR41, Chemoattractant receptor-like 2, Cmkrl2

Description: The G protein-coupled estrogen receptor (GPER), formerly known as G protein receptor 30 (GPR30), was identified as a novel estrogen receptor that mediates a rapid, non-genomic response to estrogens1.GPER is a G protein-coupled seven-transmembrane receptor. It is believed that the N-terminus is located outside of the cell, and that aspartic acid residues in the terminal region might be modified by glycosylation if GPER is localized in the plasma membrane. It is speculated that the ligand associates with the N-terminal domain to activate the receptor. Trimeric G protein is presumed to bind to the 3rd loop of the intracellular domain based on the molecular structure. A PDZ domain appears to be in the C-terminal region of GPER, but its physiological role is unknown2.GPER is widely distributed in the human body including: brain, liver, heart, lung, pancreas, placenta, blood vessels, bone, lymphoid tissue, as well as endometrial, ovarian and breast cancer. The receptor has been localized both in the plasma membrane and in the endoplasmic reticulum3.It was found that GPER is rapidly induced in the cardiomyocyte ischemia model, and that its induction is associated with apoptotic cell death during ischemia and reperfusion4. In addition, the GPER mediates estrogenic signaling in breast cancer cells and cancer-associated fibroblasts (CAF) that contribute to cancer progression5.

Gene ID: 171104

NCBI Accession: NM_001039966

UniProt: O08878

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process