GPR39 antibody (Extracellular)

Catalog No. ABIN7043159

Product Details anti-GPR39 Antibody

Target: GPR39 (G Protein-Coupled Receptor 39 (GPR39))

Binding Specificity: AA 309-320, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR39 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to GPR39

Specificity: 3rd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: rat - 9,Human - 11,12 amino acid residues identical

Characteristics: Anti-GPR39 (extracellular) Antibody (ABIN7043159, ABIN7044429 and ABIN7044430)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize GPR39 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KPKHDWTRTYFR, corresponding to amino acid residues 309-320 of mouse GPR39

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-GR045

Blocking Peptide: BLP-GR045

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR39

Target: GPR39 (G Protein-Coupled Receptor 39 (GPR39))

Alternative Name: GPR39 (GPR39 Products)

Background: G-protein coupled receptor 39, Zinc receptor GPR39,GPR39 (G-protein coupled receptor 39) is member of the rhodopsin-like family of 7-transmembrane containing GPCRs and is closely related to the ghrelin/neurotensin receptor subfamily.The human GPR39 gene encodes the full-length receptor (GPR39-1a). GPR39 is expressed in various tissues including adipose tissue, heart and thyroid but is most abundant in the pancreas, GI tract, liver and kidney. The GPR39 receptor is comprised of an extracellular N-terminus, seven membrane spanning loops, and an intracellular C-terminus. It also contains four cysteine residues in its extracellular domains. These have been shown to form two disulfide bridges which are conserved within all vertebrate GPR39 sequences with the exception that the first bridge is absent from the fish receptors.Although GPR39 has a high degree of constitutive activity mediated through Gαq and Gα12/13 pathways, it has been discovered recently that Zn2+ serves as a ligand capable of activating the receptor. Following Zinc activation, both pathways are significantly enhanced and signaling occurs through the Gαs pathway as well.GPR39 has been implicated in several pathologies: Disruption of the GPR39 gene is associated with impaired insulin secretion. GPR39 knockout mice exhibit decreased insulin levels and lower levels of insulin promoting factor-1 and HNF-1α in pancreatic islets. GPR39 knockout mice also exhibit lower levels of CREB and BDNF proteins that are important in neuronal plasticity and antidepressant response thus leading to depressive-like behaviors in animals.

Alternative names: GPR39, G-protein coupled receptor 39, Zinc receptor GPR39

Gene ID: 71111

NCBI Accession: NM_001508

UniProt: Q5U431

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Feeding Behaviour