GPR39 antibody (Extracellular Domain)

Catalog No. ABIN1048812

Product Details anti-GPR39 Antibody

Target: GPR39 (G Protein-Coupled Receptor 39 (GPR39))

Binding Specificity: Extracellular Domain

Reactivity: Human, Mouse, Rat, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR39 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human GPR39. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Horse, Rabbit (100%) Marmoset, Bovine, Panda, Turkey, Chicken (94%) Pig, Opossum (88%) Dog (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR39. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Mouse, Rat, Horse, Rabbit (100%), Marmoset, Bovine, Panda, Turkey, Chicken (94%), Pig, Opossum (88%), Dog (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (1.25 - 2.5 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

References for anti-GPR39 antibody (ABIN1048812)

Alkayed, Cao, Qian, Nagarajan, Liu, Nelson, Xie, Li, Fan, Liu, Grafe, Davis, Xiao, Barnes, Kaul: "Control of Coronary Vascular Resistance by Eicosanoids via a Novel GPCR." in: , (2022) (PubMed).

Davis, Bah, Zhang, Nelson, Golgotiu, Nie, Alkayed, Young, Woltjer, Silbert, Grafe, Alkayed: "GPR39 localization in the aging human brain and correlation of expression and polymorphism with vascular cognitive impairment." in: Alzheimer's & dementia (New York, N. Y.), Vol. 7, Issue 1, pp. e12214, (2021) (PubMed).

Target Details for GPR39

Target: GPR39 (G Protein-Coupled Receptor 39 (GPR39))

Alternative Name: GPR39 (GPR39 Products)

Synonyms: GPR39 antibody, zgc:64187 antibody, 4933415E13Rik antibody, G protein-coupled receptor 39 antibody, G-protein coupled receptor 39 antibody, Gpr39 antibody, GPR39 antibody, LOC720610 antibody, gpr39 antibody

Background: Name/Gene ID: GPR39
Subfamily: Orphan-A
Family: GPCR

Synonyms: GPR39, G protein-coupled receptor 39, G-protein coupled receptor 39

Gene ID: 2863

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Feeding Behaviour