GALR2 antibody (3rd Intracellular Loop)

Catalog No. ABIN7043181

The Rabbit Polyclonal anti-GALR2 antibody (ABIN7043181) specifically detects GALR2 in WB, IHC and IF. The antibody is reactive with Mouse samples.

Quick Overview for GALR2 antibody (3rd Intracellular Loop) (ABIN7043181)

Target: GALR2 (Galanin Receptor 2 (GALR2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GALR2 Antibody

Binding Specificity: 3rd Intracellular Loop, AA 223-235

Purpose: A Rabbit Polyclonal Antibody to Galanin Receptor Type 2

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat - identical, human - 11,13 amino acid residues identical

Characteristics: Anti-GALR2 Antibody is directed against an epitope of the mouse Galanin receptor 2. Anti-GALR2 Antibody (ABIN7043181, ABIN7044398 and ABIN7044399) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GALR2 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GSGSQRAKRKVTR, corresponding to amino acid residues 223-235 of mouse Galanin receptor type 2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235477)

Blocking Peptide: (ABIN7235477)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for GALR2

Target: GALR2 (Galanin Receptor 2 (GALR2))

Alternative Name: GALR2

Background:

Synonyms: Galanin receptor type 2, GALNR2, GAL2R

Description: Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.1-4GALR2 is widely expressed in the brain (with highest levels of mRNA found in the hypothalamus, hippocampus, amygdala and pyriform cortex) and in peripheral tissues such as the small intestine, heart, kidney, and liver. High levels of GALR2 expression were also found in rat dorsal root ganglia.1GALR2 activation increases inositol phosphate hydrolysis, mediates the release of Ca2+ into the cytoplasm from intracellular stores, and opens Ca2+-dependent chloride channels in a PTX-resistant manner, indicating that GALR2 couples to Gq/11 proteins.5

Gene ID: 14428

NCBI Accession: NM_003857

UniProt: O88854

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Feeding Behaviour