GFRA4 antibody (Extracellular)

Catalog No. ABIN7043213

Product Details anti-GFRA4 Antibody

Target: GFRA4 (GDNF Family Receptor alpha 4 (GFRA4))

Binding Specificity: AA 160-172, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFRA4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to GDNF Family Receptor α4

Specificity: Extracellular, N-terminus

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for human samples. The antibody will recognize both the membrane bound canonical isoform and the putative secreted isoform.

Predicted Reactivity: mouse - 11,Rat - 12,13 amino acid residues identical

Characteristics: Anti-GFR alpha 4 (extracellular) Antibody is directed against an extracellular epitope of rat GDNF receptor α4. Anti-GFR alpha 4 (extracellular) Antibody (ABIN7043213, ABIN7044781 and ABIN7044782) can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GFR α4 from mouse and rat samples. The antibody is not suitable for detecting GFR α4 from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GSRDG(S)PEEGGPR, corresponding to amino acid residues 160-172 of rat GFRA4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:800

Comment:

Negative Control: (ABIN7235584)

Blocking Peptide: (ABIN7235584)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GFRA4

Target: GFRA4 (GDNF Family Receptor alpha 4 (GFRA4))

Alternative Name: GFRA4 (GFRA4 Products)

Background: GFRA4, GFRα4, GDNF family receptor α4, GDNF receptor alpha 4, Persephin receptor,The GDNF family ligands (GFLs) belong to the super family of the TGF-β. They belong to the group of cystine-knot protein and function as homodimers1. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)2.These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis1.In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected1,3. Also alternative spliced forms of the protein can lead to soluble forms of GFRα receptors3.

Alternative names: GFR alpha 4, GFRA4, GFRalpha4, GDNF family receptor alpha4, GDNF receptor alpha 4, Persephin receptor

Gene ID: 66023

NCBI Accession: NM_145762

UniProt: Q9EPI2