GRIN1/NMDAR1 antibody (Extracellular)

Catalog No. ABIN7043243

The Guinea Pig Polyclonal anti-GRIN1/NMDAR1 antibody has been validated for WB, IHC and IF. It is suitable to detect GRIN1/NMDAR1 in samples from Rat.

Quick Overview for GRIN1/NMDAR1 antibody (Extracellular) (ABIN7043243)

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This GRIN1/NMDAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-GRIN1/NMDAR1 Antibody

Binding Specificity: Extracellular

Purpose: A Guinea Pig Polyclonal Antibody to NMDA Receptor 1 (GluN1)

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Human,mouse - identical

Characteristics: Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody is directed against an extracellular epitope of rat NMDA Receptor 1. Guinea pig Anti-NMDAR1 (GluN1) (extracellular) Antibody  raised in guinea pig can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting NMDAR1 in living cells. It has been designed to recognize NMDAR1 from rat, mouse and human samples. The antigen used to immunize guinea pigs is the same as Anti-NMDAR1 (GluN1) (extracellular) Antibody (ABIN7043242, ABIN7044322 and ABIN7044323) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: ETEKPRGYQMSTRLK(C), corresponding to amino acid residues 385-399 of rat GluN1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600-1:1200

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235673)

Blocking Peptide: (ABIN7235673)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: GRIN1

Background: NMDA receptor 1, Ionotropic glutamate receptor subunit ζ 1, N-methyl-D-aspartate receptor subunit 1, GRIN1,The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors.The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit.The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels.Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson's and Alzheimer's disease.NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

Alternative names: NMDAR1 (GluN1), NMDA receptor 1, Ionotropic glutamate receptor subunit zeta1, N-methyl-D-aspartate receptor subunit 1, GRIN1, GLUN1

Gene ID: 24408

NCBI Accession: NM_007327

UniProt: P35439

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity