HRH2 antibody (Extracellular)

Catalog No. ABIN7043270

Product Details anti-HRH2 Antibody

Target: HRH2 (Histamine Receptor H2 (HRH2))

Binding Specificity: AA 161-173, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunochromatography (IC), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Histamine H2 Receptor

Specificity: 2nd extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Will not recognize human histamine H2 receptor.

Predicted Reactivity: Mouse - identical

Characteristics: Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody is directed against an epitope of rat histamine H2 receptor. Anti-Histamine H2 Receptor (HRH2) (extracellular) Antibody (ABIN7043270, ABIN7044507 and ABIN7044508)) can be used in western blot, indirect flow cytometry, immunohistochemistry, and immunocytochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize H2R from rat and mouse samples. It will not recognize human H2R.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat HRH2 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-HR002

Blocking Peptide: BLP-HR002

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HRH2

Target: HRH2 (Histamine Receptor H2 (HRH2))

Alternative Name: HRH2 (HRH2 Products)

Background: H2R, HH2R, Gastric receptor I, Gastric receptor 1,Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1,2The biological effects of histamine are mediated through four receptors (H1-H4 receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.H2 receptors couple to adenylate cyclase via Gs, leading to the formation of cAMP and the activation of protein kinase A (PKA).1,2 H2 receptors are expressed in several tissues including the brain, gastrointestinal tract and heart.The most studied physiological role of the H2 receptor is its involvement in the regulation of gastric acid secretion.3 Specific H2 receptor antagonists are currently used for the treatment of acid-related gastrointestinal conditions such as peptic ulcer, gastroesophageal reflux disease and dyspepsia.Involvement of H2 receptors in chronic heart failure and immune regulation has also been suggested.

Alternative names: Histamine H2 Receptor (HRH2), H2R, HH2R, Gastric receptor I, Gastric receptor 1

Gene ID: 25461

NCBI Accession: NM_001131055

UniProt: P25102