MANF antibody (AA 91-104) (Atto 594)

Catalog No. ABIN7043331

The Atto 594-conjugated Rabbit Polyclonal anti-MANF antibody (ABIN7043331) specifically detects MANF in IHC and IF. The antibody is reactive with Human samples.

Quick Overview for MANF antibody (AA 91-104) (Atto 594) (ABIN7043331)

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MANF antibody is conjugated to Atto 594

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-MANF Antibody (Atto 594)

Binding Specificity: AA 91-104

Purpose: A Rabbit Polyclonal Antibody to MANF Conjugated to the Fluorescent Dye ATTO-594

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-MANF/ARMET Antibody (ABIN7043330, ABIN7044787 and ABIN7044788) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MANF from human, rat, and mouse samples. \nAnti-MANF/ARMET-ATTO Fluor-594 Antibody (ABIN7043331) is directly labeled with an fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-MANF/ARMET-ATTO Fluor-594 Antibody is especially suited to experiments requiring simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EVSKPLAHHIPVEK, corresponding to amino acid residues 91-104 of human MANF

Isotype: IgG

Application Details

Application Notes:

WB: The optimal concentration should be determined by the user

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:80

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7235952)

Blocking Peptide: (ABIN7235952)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for MANF

Target: MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Alternative Name: MANF

Background:

Synonyms: Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP

Description: The novel protein mesencephalic astrocyte-derived neurotrophic factor (MANF) is a survival molecule (alternatively referred to as arginine-rich, mutated in early stage tumors, or ARMET) that has high selectivity for dopaminergic neurons of the midbrain1,2. There, it induces proliferative and protective effects against neurodegeneration (usually caused by 6-hydroxydopamine), its therapeutic potential in dopamine-related neurodegenerative disorders such as Parkinson's disease is therefore promising3,4. MANF is also expressed cardiac tissues. As was recently suggested, it induces cardioprotection in the heart and anti-hypertrophic effects via reducing endoplasmic reticulum stress in ischemic conditions in both the heart and the brain - thus, its effects stretch beyond rescue and protection of dopaminergic neurons alone1,5.Generally, neurotrophic factors (NTFs) provide support, protection, and regulation for neurons - with each group of neurons having their own unique NTFs - both during development and at adulthood. Other noticeable NTFs include neurotrophin-3 and -4 (NT3 and NT4), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), and glial cell derived neurotrophic factor (GDNF)6.MANF is considered to be evolutionary conserved within Mammalia and beyond (including distant species such as nematode and fruit fly - the latter shares more than 50 % identity with human MANF gene) and is highly mutative in many tumors. It is mostly composed of alpha helices and random coils, its structure essentially differs from any other neurotrophic factor. It is suggested that the protein initiates intracellular signaling cascade by binding to cell-surface lipids and membranes via its cysteine-rich N-terminal. Together with its C-terminal which contains a disulfide bridge that endows a cytoprotective response against ER stress, MANF is implied to be bi-functional3,7.

Gene ID: 7873

NCBI Accession: NM_006010

UniProt: P55145