NGFR antibody (Soluble) (FITC)

Catalog No. ABIN7043343

This anti-NGFR antibody is a Mouse Monoclonal antibody detecting NGFR in FACS. Suitable for Rat.

Quick Overview for NGFR antibody (Soluble) (FITC) (ABIN7043343)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 192-IgG

Product Details anti-NGFR Antibody (FITC)

Binding Specificity: Soluble

Purpose: A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR Conjugated to the Fluorescent Dye FITC

Cross-Reactivity (Details): 192-IgG reacts with rat p75NTR. It does not cross-react with p75NTR of human or mouse origin.

Predicted Reactivity: Recognizes p75NTR from rat samples only

Characteristics: Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (ABIN7043342, ABIN7044624, ABIN7044625 and ABIN7044626) is a highly specific monoclonal antibody directed against p75NTR. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize p75NTR from rat samples only. \nMouse Anti-Rat p75 NGF Receptor (extracellular)-FITC Antibody (ABIN7043342, ABIN7044624, ABIN7044625 and ABIN7044626-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody was specifically developed to be used in direct flow cytometry applications, especially for those that use multiple and simultaneous labeling of different markers.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Solubilized proteins from PC12 membranes.

Immunogen Sequence: Solubilized proteins from PC12 membranes.

Isotype: IgG1

Application Details

Application Notes:

Antigen preadsorption control: NA

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR

Background: P75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271,p75NTR known as a cell surface receptor for NGF, BDNF, NT-3 and NT-4, is also a receptor for β-amyloid, and for the myelin axon repellant protein MAG.1,2,4,6 Binding of neurotrophins induces receptor dimerization followed by phosphorylation of receptor kinase residues and recruitment of intracellular proteins involved in signal transduction. p75NTR functions alone, or in functional complexes with the neurotrophin receptors trkA, trkB and trkC. p75NTR signals via interaction with different transducers such as TRAF6, IRAK, and RIP-2, activating NF-κB, JNK and p38, and inactivating rhoA.1,3,6 Receptor degradation is mediated by a metalloproteinase-dependent shedding of the extracellular domain. p75NTR binds all neurotrophins with similar nM affinity, but binds pro-neurotrophins with greater affinity, and is more potently activated by pro-neurotrophins.5 Overexpression of the intracellular domain in developing neurons induces apoptosis. NGF-dependent association of NRIF with p75NTR contributes to pro-apoptotic signaling.7,8 β-amyloid peptide binds p75NTR and stimulates pro-apoptotic signaling by p75NTR, suggesting that the receptor may be relevant for Alzheimer's disease.9

Alternative names: p75 NGF Receptor, p75NTR, NGFR, CD271, TNFR superfamily member 16

Gene ID: 24596

NCBI Accession: NM_002507

UniProt: Q08201

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding