Neuregulin 1 antibody (Extracellular)

Catalog No. ABIN7043353

This Rabbit Polyclonal antibody specifically detects Neuregulin 1 in WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for Neuregulin 1 antibody (Extracellular) (ABIN7043353)

Target: Neuregulin 1 (NRG1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neuregulin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Neuregulin 1 Antibody

Binding Specificity: AA 87-101, Extracellular

Purpose: A Rabbit Polyclonal Antibody to Neuregulin-1

Specificity: Extracellular, N-terminus

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): This antibody will recognize Neuregulin-1 Type I, Type II and Type IV. It will not recognize Neuregulin-1 Type III.

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-NRG1 (Neuregulin-1) (extracellular) Antibody (ABIN7043353, ABIN7044718 and ABIN7044719) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and could potentially detect the protein in living cells. It has been designed to recognize Neuregulin-1 from rat, human, and mouse samples. This antibody will recognize NRG1 Type I, Type II and Type IV. It will not recognize Neuregulin-1 Type III.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KIQKKPGKSELRINK, corresponding to amino acid residues 87-101 of human Neuregulin-1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236026)

Blocking Peptide: (ABIN7236026)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Neuregulin 1

Target: Neuregulin 1 (NRG1)

Alternative Name: NRG1

Background: Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF,ErbB receptors are a family of tyrosine kinase receptors encoded by the erbB gene. These receptors commence signaling pathways such as the mitogen activated protein kinase (MAPK) and inositol 3-kinase (PI3K) pathways and have an important role in the control of cell growth and survival1. Neuregulins are a family of EGF-like factors that are produced from alternative RNA splicing of the same gene. Through their interaction with ErbB receptors, Neuregulins regulate normal developmental processes, plasticity, and oncogenesis2.Neuregulin-1 is one of the four known proteins of the Neuregulin family and can be found in many organs including the heart, breast, peripheral and central nervous system. Neuregulin-1 is encoded by a gene located on chromosome 8p13 which produces more than 15 different isoforms of the same protein. All isoforms contain a core EGF domain encoded by exon E130 but other elements of the protein vary. Most NRG1 isoforms are synthesized as transmembrane proproteins which undergo proteolytic cleavage processes3.Due to its important function in the central and peripheral nervous system as well as in other organs NRG1 has been implicated in a variety of pathologies. The NRG1 gene is suspected as a schizophrenia susceptibility gene. Mice with a mutated NRG1 exhibit elevated activity in open field and abnormal sensorimotor gating which correspond with schizophrenic like behavior in humans. These affects are reversed using anti-psychotic drugs4. In addition, Activation of NRG1 was found to improve cardiac survival and function in a rat and canine model of dilated and ischemic hearts5.

Alternative names: NRG1 (Neuregulin-1) , Glial growth factor, GGF, Heregulin, HRG, Sensory and motor neuron derived factor, SMDF, Neu differentiation factor, NDF

Gene ID: 3084

NCBI Accession: NM_001159995

UniProt: Q02297

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation