NPY5R antibody (AA 291-306)

Catalog No. ABIN7043375

Product Details anti-NPY5R Antibody

Target: NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

Binding Specificity: AA 291-306

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPY5R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Purpose: A Rabbit Polyclonal Antibody to Neuropeptide Y5 Receptor

Specificity: 3rd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse,bovine,canis - identical, human - 15,16 amino acid residues identical

Characteristics: Anti-NPY5R Antibody is directed against an epitope of the rat neuropeptide Y5 receptor (NPY5R). Anti-NPY5R Antibody (ABIN7043375 and ABIN7044673)) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize NPY5R from rat, human and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: SFIRKHRRRYSKKTAC, corresponding to amino acid residues 291-306 of rat NPY5R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: BLP-NR025

Blocking Peptide: BLP-NR025

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NPY5R

Target: NPY5R (Neuropeptide Y Receptor Y5 (NPY5R))

Alternative Name: NPY5R (NPY5R Products)

Background: Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5,Neuropeptide Y (NPY) is a 36 amino acid peptide neurotransmitter present in the central and peripheral nervous systems.1 NPY is involved in the regulation of a broad range of functions such as eating behavior, hypertension, and reproduction.2NPY exerts its effects via five receptor subtypes known as the neuropeptide Y receptors (NPYR): Y1 (NPY1R), Y2 (NPY2R), Y4 (NPY4R), Y5 (NPY5R), and Y6 (NPY6R).2 Four of these (Y1, Y2, Y4, and Y5) are expressed in humans.2 The NPY receptors belong to the G protein-coupled receptor superfamily whose members share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Activation of these receptors by NPY affects [Ca2+]i, adenylate cyclase signalling, and a number of ion channel functions.3 Based on their high frequency and density in steroid hormone-producing tumors, the NPYRs might be of potential use in tumor management.2NPY5R as well as NPY1R and NPY2R are expressed in the hypothalamus, in the brain stem, and in peripheral tissues, such as blood vessels, lung, kidney, adrenal glands, stomach, colon, heart, pancreas, and intestine. NPY5R has been postulated as the mediator of the feeding response to NPY and related peptides (125 in Kamiji, M. M). Its localization in the brain is consistent with this hypothesis.4

Alternative names: NPY5R, Neuropeptide Y5 receptor, Neuropeptide Y receptor type 5, NPY Y5 receptor, NPYY5

Gene ID: 25340

NCBI Accession: NM_006174

UniProt: Q63634

Pathways: Feeding Behaviour