P2RY2 antibody (C-Term, Extracellular)

Catalog No. ABIN7887023

The Rabbit Polyclonal anti-P2RY2 antibody (ABIN7887023) specifically detects P2RY2 in WB, IHC, IF, FACS, IC and LCI. The antibody is reactive with Rat samples.

Quick Overview for P2RY2 antibody (C-Term, Extracellular) (ABIN7887023)

Target: P2RY2 (Purinergic Receptor P2Y, G-Protein Coupled, 2 (P2RY2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P2RY2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunochromatography (IC), Live Cell Imaging (LCI)

Product Details anti-P2RY2 Antibody

Binding Specificity: AA 11-24, C-Term, Extracellular

Purpose: A Rabbit Polyclonal Antibody to P2Y2 Receptor

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat, human - 13,mouse - 14,15 amino acid residues identical

Characteristics: Anti-P2Y2 Receptor (extracellular) Antibody (ABIN7043424, ABIN7045138 and ABIN7045139) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, live cell imaging, and immunohistochemistry applications. It has been designed to recognize P2RY2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (CD)TINGTWEGDELGYK, corresponding to amino acid residues 11- 24 of rat P2RY2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: ICC

Negative Control: (ABIN7236218)

Blocking Peptide: (ABIN7236218)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for P2RY2

Target: P2RY2 (Purinergic Receptor P2Y, G-Protein Coupled, 2 (P2RY2))

Alternative Name: P2RY2

Background:

Synonyms: P2Y purinoceptor 2, P2RY2, P2U1

Description: P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation1.The mammalian P2Y family currently includes eight functional receptors, P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y142-4.The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway5. It has become a leading target for the treatment of cystic fibrosis. Activation of P2RY2 increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis.6

Gene ID: 29597

NCBI Accession: NM_176072

UniProt: P41232

Pathways: Cellular Response to Molecule of Bacterial Origin, Smooth Muscle Cell Migration