PIEZO1 antibody (AA 1863-1876)

Catalog No. ABIN7043434

Product Details anti-PIEZO1 Antibody

Target: PIEZO1 (Piezo-Type Mechanosensitive Ion Channel Component 1 (PIEZO1))

Binding Specificity: AA 1863-1876

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIEZO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Piezo1 Channel

Specificity: The peptide is located in an internal region of the rat Piezo1 (the exact topology of Piezo1 remains to be defined).

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Not recommended for use with human samples.

Predicted Reactivity: Mouse - 12,14 amino acid residues identical

Characteristics: Anti-Piezo1 Antibody (ABIN7043434, ABIN7044988 and ABIN7044989) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Piezo1 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Purification: Affinity purified on immobilized antigen.

Grade: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EDLKPQHRRHISIR, corresponding to amino acid residues 1863-1876 of rat Piezo1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:600

Comment:

Cited Application: IP|IHC|ICC|LCI

Negative Control: (ABIN7236252)

Blocking Peptide: (ABIN7236252)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PIEZO1

Target: PIEZO1 (Piezo-Type Mechanosensitive Ion Channel Component 1 (PIEZO1))

Alternative Name: PIEZO1 (PIEZO1 Products)

Background: Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment,Piezo1 protein is a family member of mixed cationic, non selective, directly mechanically activated ion channels found in higher eukaryotic cells. Piezo proteins are associated with physiological responses in cells that have been identified in many cell types and tissues in several species where mechanosensitivity plays a major role1,2.Two mammalian Piezo proteins were identified, piezo1 and piezo2 encoded by the fam38A and fam38B genes recpectively2. Piezo1 expression is detected in organs such as: bladder, colon, kidney, lung and skin3.The secondary structure and general length of Piezo proteins are quite conserved among species, and seem to form homotetrameric aggregates. Each subunit comprises between 24-32 putative transmembrane domains. The channels are constitutively active in liposomes and their activity does not require any other proteins1.Mechanosensitive proteins have been linked to various pathophysiological situations such as hearing and deafness, cardiac physiological and hypertrophic responses, and kidney function and disease2.Several studies have demonstrated that Piezo1 is essential for the removal of extra cells without apoptosis. Mutations in Piezo1 protein have been linked to the pathological response of red blood cells in a genetic disease called Xerocytosis. These finding suggest that Piezo1 plays a major role in cells' responses to mechanical stimuli1. In addition, Piezo1 plays a critical role in endothelial cell mechanotransduction which is essential for embryonic development. Knock-out model of Piezo1 affects the ability of endothelial cells to alter their alignment when exposed to shear stress. Furthermore, loss of Piezo1 in endothelial cells leads to deficits in stress fiber and cellular orientation in response to shear stress, therefore Piezo1mechanotransduction is linked to regulation of cell morphology4.

Alternative names: Piezo1, Piezo-type mechanosensitive ion channel component 1, Protein FAM38A, Mib, Membrane protein induced by beta-amyloid treatment

Gene ID: 361430

NCBI Accession: NM_014745

UniProt: Q0KL00