KCNK18 antibody (Extracellular)

Catalog No. ABIN7043446

Product Details anti-KCNK18 Antibody

Target: KCNK18 (Potassium Channel Subfamily K Member 18 (KCNK18))

Binding Specificity: AA 62-75, Extracellular

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNK18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to KCNK18 Channel

Specificity: 1st extracellular loop

Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Unlikely to recognize human samples.

Predicted Reactivity: Rat - 13,14 amino acid residues identical

Characteristics: Anti-KCNK18 (TRESK) (extracellular) Antibody is directed against an epitope of mouse KCNK18. Anti-KCNK18 (TRESK) (extracellular) Antibody (ABIN7043446, ABIN7045021 and ABIN7045022) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize TRESK channel from mouse and rat samples. The antibody will unlikely recognize TRESK from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7236295)

Blocking Peptide: (ABIN7236295)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNK18

Target: KCNK18 (Potassium Channel Subfamily K Member 18 (KCNK18))

Alternative Name: KCNK18 (KCNK18 Products)

Background: Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1,KCNK18 (also named TWIK-related spinal cord K+ channel, TRESK or K2P18.1) is a member of the 2-pore (2P) domain K+ channel family that currently includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation.1The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. It has been proposed that the functional channel unit is a dimer.Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH , mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc.KCNK18 is the only K2P channel, known so far, whose current is activated following Gαq-receptor coupled activation. The enhancement of KCNK18 current involves activation of calcineurin (calcium-calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gαq activation.2 In addition, KCNK18 is potently activated by clinical concentrations of volatile anesthetics.3KCNK18 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen.KCNK18 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia.

Alternative names: KCNK18 (TRESK), Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1

Gene ID: 332396

NCBI Accession: NM_181840

UniProt: Q6VV64