KCNN4 antibody (Intracellular)

Catalog No. ABIN7043465

The Rabbit Polyclonal anti-KCNN4 antibody is suitable to detect KCNN4 in samples from Rat. It has been validated for WB, IHC, IF, IC and IP.

Quick Overview for KCNN4 antibody (Intracellular) (ABIN7043465)

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KCNN4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Immunoprecipitation (IP)

Product Details anti-KCNN4 Antibody

Binding Specificity: AA 350-363, Intracellular

Purpose: A Rabbit Polyclonal Antibody to KCNN4 (KCa3.1, SK4) Channel

Specificity: Intracellular, C-terminal domain

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human,pig - identical

Characteristics: Anti-KCNN4 (KCa3.1, SK4) Antibody (ABIN7043465, ABIN7044979 and ABIN7044980) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis, immunocytochemistry, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize KCNN4 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: RQVRLKHRKLREQV(C), corresponding to amino acid residues 350-363 of rat KCNN4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IP|IHC|ICC

Negative Control: (ABIN7236353)

Blocking Peptide: (ABIN7236353)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KCNN4

Target: KCNN4 (Potassium Intermediate/small Conductance Calcium-Activated Channel, Subfamily N, Member 4 (KCNN4))

Alternative Name: KCNN4

Background: IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel,KCa3.1 (KCNN4, SK4) is a member of the Ca2+ activated K+ channel family that shares the characteristic of being activated by intracellular Ca2+. The channel has an intermediate conductance, is voltage insensitive and is activated by Ca2+ in the submicromolar range. The channel has a similar topology to that of KV channels, that is, six transmembrane domains and intracellular N- and C-termini. KCa3.1 is widely expressed in epithelial, endothelial and cells of hematopoietic origin. In erythrocytes (red blood cells) it has been identified as the molecular correlate of the so-called Gardos channel. The functional role of the channel is to set the cell membrane potential at negative values so as to aid in the electrochemical transport of other ions such as Cl- and Ca2+. Indeed, KCa3.1 has a key role in sustaining the Ca2+ influx in activated T lymphocytes and in regulating Cl- secretion from colon epithelium. Therefore, specific blockers of the KCa3.1 channel have been proposed for the treatment of several diseases including autoimmune diseases, secretory diarrhea and sickle cell anemia.

Alternative names: KCNN4 (KCa3.1, SK4), IKCa1, IK1, Intermediate conductance Ca2+-activated K+ channel protein 4, Gardos channel

Gene ID: 65206

NCBI Accession: NM_002250

UniProt: Q9QYW1