KCNJ3 antibody (Extracellular Loop)

Catalog No. ABIN7044555

The Mouse Monoclonal anti-KCNJ3 antibody is suitable to detect KCNJ3 in samples from Human. It has been validated for WB, IF, IC and LCI.

Quick Overview for KCNJ3 antibody (Extracellular Loop) (ABIN7044555)

Target: KCNJ3 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 3 (KCNJ3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNJ3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Clone: 3C3

Product Details anti-KCNJ3 Antibody

Binding Specificity: Extracellular Loop

Purpose: A Mouse Monoclonal Antibody to GIRK1 (Kir3.1) Channel

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Kir3.1 from human,rat,mouse samples

Characteristics: Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody (ABIN7043483 and ABIN7044555) is a highly specific mouse monoclonal antibody directed against an epitope of the human protein. The antibody can be used in western blot, and immunocytochemistry applications. It has been designed to recognize Kir3.1 from human, rat and mouse samples.

Purification: Affinity purified from cultured hybridoma medium.

Immunogen: Synthetic peptide mapping to the extracellular loop of human GIRK1 

Isotype: IgG1

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:20

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL or 100 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for KCNJ3

Target: KCNJ3 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 3 (KCNJ3))

Alternative Name: KCNJ3

Background:

Synonyms: KCNJ3, G protein-activated inward rectifier potassium channel 1

Description: Kir3.1 (or G-protein regulated inward-rectifier K+ channel 1, GIRK1) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heterotetramers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir3.1 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Gα-GTP from the Gβγ dimer. The latter directly binds to Kir3 and activates the channel1,3.In the heart, Kir3.1 co-assembles with Kir3.4 to form the prototypical muscarinic-gated K+ channel KAch current, responsible for slowing the heart rate in response of parasympathetic stimulation2.In the brain, Kir3.1 co-assembles with Kir3.2 and mediates the inhibitory effects of many neurotransmitters including opioid, adrenergic, muscarinic, dopaminergic and γ-aminobutyric acid (GABA)1,3.A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (#STT-250) was shown to be a potent blocker of Kir3.1 containing channels (8.6 nM for the Kir3.1/3.4 combination and 5.4 nM for the Kir3.1/3.2)4,5.

Gene ID: 3760

NCBI Accession: NM_002239

UniProt: P48549