Prostacyclin Receptor antibody (Extracellular)

Catalog No. ABIN7043563

Product Details anti-Prostacyclin Receptor Antibody

Target: Prostacyclin Receptor (PTGIR)

Binding Specificity: AA 290-302, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Prostacyclin Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Purpose: A Rabbit Polyclonal Antibody to Prostacyclin Receptor (PTGIR)

Specificity: 3rd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 11,13 amino acid residues identical

Characteristics: Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody is directed against an epitope of rat the PGI2 receptor. Anti-Prostacyclin Receptor (PTGIR) (extracellular) Antibody (ABIN7043563, ABIN7045122 and ABIN7045123)) can be used in western blot analysis. The antibody recognizes an extracellular epitope, ideal for detecting the receptor in living cells. It has been designed to recognize PGI2 receptor from human, mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)TQAIAPDSREMGD, corresponding to amino acid residues 290-302 of rat PTGIR

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Comment:

Negative Control: BLP-PR068

Blocking Peptide: BLP-PR068

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Prostacyclin Receptor

Target: Prostacyclin Receptor (PTGIR)

Alternative Name: PTGIR (PTGIR Products)

Background: Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor,Prostaglandins comprise a family of locally acting hormones, whose synthesis is initiated by the enzyme COX. This pathway is involved in a broad variety of pathological conditions such as cancer, inflammation and hypertension.Prostaglandin I2 receptor (PGI2 receptor) is G-coupled protein receptor (GPCR) with seven hydrophobic transmembrane segments and an extracellular NH2 terminus. Prostaglandin receptors belong to family A, group V of GPCRs. There is significant ligand-binding cross-reactivity between different prostaglandins and different receptors in the family. Within the PG family, receptors share roughly 20-30 % of their sequence and there are 34 identical residues across the family. Twenty of these residues are unique to PG receptors and are not shared by other GPCRs. The unique residues reside mostly in the transmembrane region and in the second extracellular loop region. Both of these regions are implicated in ligand binding. The PGI2 receptor signals primarily via stimulation of cAMP generation but through PIP2 as well despite a much higher agonist concentration required for PIP2 hydrolysis.PGI2 receptor mRNA is highly expressed in the human heart, aorta, kidney, liver and lung tissues and in mice thymus, heart and spleen1. Mice lacking PGI2 receptor have been shown to have a reduced inflammation response and a significant decrease in clinical arthritis. In addition, human patients with a dysfunctional PGI2 receptor variant exhibit an enhanced atherothrombotic phenotype with higher incidence of triple vessel coronary disease and cardiovascular events2.

Alternative names: Prostacyclin Receptor (PTGIR), Prostanoid IP receptor, Prostaglandin I2 receptor, PGI2 receptor

Gene ID: 292661

NCBI Accession: NM_000960

UniProt: P43253

Pathways: cAMP Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling