RYR2 antibody (Intracellular, N-Term)

Catalog No. ABIN7887081

This Rabbit Polyclonal antibody specifically detects RYR2 in IHC, WB, IF and IC. It exhibits reactivity toward Human.

Quick Overview for RYR2 antibody (Intracellular, N-Term) (ABIN7887081)

Target: RYR2 (Ryanodine Receptor 2 (Cardiac) (RYR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RYR2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-RYR2 Antibody

Binding Specificity: AA 1489-1502, Intracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Ryanodine Receptor 2

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse - identical

Characteristics: Anti-Ryanodine Receptor 2 Antibody (ABIN7043605, ABIN7045213 and ABIN7045214) is a highly specific antibody directed against an epitope of human RyR2. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize RyR2 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: CAGESMSPGQGRNN, corresponding to amino acid residues 1489-1502 of human RyR2

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:50-1:100

IP: The optimal concentration should be determined by the user

Comment:

Cited Application: IP|IHC|ICC

Negative Control: (ABIN7236742)

Blocking Peptide: (ABIN7236742)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for RYR2

Target: RYR2 (Ryanodine Receptor 2 (Cardiac) (RYR2))

Alternative Name: RYR2

Background:

Synonyms: RyR2, Cardiac muscle ryanodine receptor

Description: It is well established that cytosolic calcium (Ca2+) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The primary intracellular Ca2+ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.The ER and SR contain two Ca2+ release channel families, the inositol trisphosphate receptors (IP3Rs) and the ryanodine receptors (RyRs).3The ryanodine receptor family consists of three different isoforms: the skeletal muscle isoform, ryanodine receptor type 1 (RyR1), the cardiac muscle isoform, ryanodine receptor type 2 (RyR2) and the brain isoform, ryanodine receptor type 3 (RyR3).3The ryanodine receptors are homotetrameric proteins, which upon assembly create a Ca2+ conducting channel. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of ryanodine (a poisonous alkaloid found in the South American plant Ryania speciosa) to the ryanodine receptor causes two major changes in the channel: a reduction in single-channel conductance and a marked increase in the open state probability, leading to an overall increase/decrease in the Ca2+ release capability of the channel.RyR2 serves as an intracellular Ca2+ channel in the SR membrane. It is predominantly expressed in cardiac muscle where it plays a central role in cardiac excitation-contraction coupling. RyR2 is also expressed in the brain.1-4

Gene ID: 6262

NCBI Accession: NM_001035

UniProt: Q92736

Pathways: Myometrial Relaxation and Contraction