Serotonin Receptor 3A antibody (AA 342-355)

Catalog No. ABIN7043621

Product Details anti-Serotonin Receptor 3A Antibody

Target: Serotonin Receptor 3A (HTR3A)

Binding Specificity: AA 342-355

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 3A

Specificity: 2nd intracellular loop

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 13,14 amino acid residues identical

Characteristics: Anti-5HT3A Receptor (HTR3A) Antibody (ABIN7043621, ABIN7045283 and ABIN7045284)) is a highly specific antibody directed against an epitope of rat Serotonin receptor 3A. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize 5-HT3A from mouse, human, and rat samples. 

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RPVPDWLRHLVLDR, corresponding to amino acid residues 342-355 of rat 5-Hydroxytryptamine receptor 3A

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Comment:

Cited Application: IHC

Negative Control: BLP-SR031

Blocking Peptide: BLP-SR031

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Serotonin Receptor 3A

Target: Serotonin Receptor 3A (HTR3A)

Alternative Name: HTR3A (HTR3A Products)

Background: 5-Hydroxytryptamine receptor 3A, Serotonin receptor 3A, Serotonin-gated ion channel receptor 3A,5-Hydroxytryptamine receptor 3A (5-HT3A, HTR3A) belongs to the superfamily of ligand-gated ion channels. Serotonin receptors, other than the 5-HT3 subtype, belong to the superfamily of G-protein coupled receptors.The 5-HT3 receptor is formed by five subunits arranged around a pore forming unit. Receptors could be either monomeric, such as 5-HT3A or heteromeric entities like 5-HT3A/B. Indeed, the type of channel formed displays different pharmacological and electrophysiological characteristics1,2. To date, five 5-HT3 subunits have been identified and labeled 5-HT3A-E, which show variability in the N-terminus and in the transmembrane region2. 5-HT3A and 5-HT3B are the best characterized among the different types.In general, 5-HT3 receptors are located in the peripheral and central nervous system, in lymphocytes and intestinal enterochromaffine cells2. In presynaptic neurons, activation of these receptors leads to an increase in intracellular Ca2+ (by both influx and mobilization of intracellular stores), and modulates the release of a number of neurotransmitters and neuropeptides2. At the postsynaptic level, activation leads to membrane depolarization2,3. The 5-HT3A subunit is expressed in GABAergic and enkephalinergic neurons in the spinal dorsal horn thereby marking its possible antinociceptive effect4.5-HT3 receptors have become important targets for which to develop treatments for irritable bowel syndrome (IBS), side effects resulting from chemotherapeutic treatment, schizophrenia and bipolar disorder1,2.

Alternative names: 5HT3A Receptor (HTR3A), 5-Hydroxytryptamine receptor 3A, Serotonin receptor 3A, Serotonin-gated ion channel receptor

Gene ID: 79246

NCBI Accession: NM_000869

UniProt: P35563

Pathways: Synaptic Membrane