Serotonin Receptor 4 antibody (Extracellular) (FITC)

Catalog No. ABIN7043625

This anti-Serotonin Receptor 4 antibody is a Rabbit Polyclonal antibody detecting Serotonin Receptor 4 in FACS and LCI. Suitable for Rat.

Quick Overview for Serotonin Receptor 4 antibody (Extracellular) (FITC) (ABIN7043625)

Target: Serotonin Receptor 4 (HTR4)

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 4 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-Serotonin Receptor 4 Antibody (FITC)

Binding Specificity: AA 168-180, Extracellular

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 4 Conjugated to the Fluorescent Dye FITC

Specificity: 2nd extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - 12,13 amino acid residues identical, human - 11

Characteristics: Anti-5HT4 Receptor (HTR4) (extracellular) Antibody (ABIN7043624, ABIN7045290 and ABIN7045291) is a highly specific antibody directed against an epitope of the rat Serotonin receptor 4. The antibody can be used in western blot, immunocytochemistry, and live cell imaging applications.  It has been designed to recognize 5HT4 receptor from rat, mouse, and human samples. \nAnti-5HT4 Receptor (HTR4) (extracellular)-FITC Antibody (ABIN7043624, ABIN7045290 and ABIN7045291)-F) is directly conjugated to fluorescein isothiocyanate (FITC). The antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DVIEKRKFNHNSN, corresponding to amino acid residues 168-180 of rat 5-Hydroxytryptamine receptor 4

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Serotonin Receptor 4

Target: Serotonin Receptor 4 (HTR4)

Alternative Name: HTR4

Background: 5-Hydroxytryptamine receptor 4, Serotonin receptor 4,5-hydroxytryptamine receptor 4 (5-HT4 receptor) is a subclass among the large family of receptors mediating serotonin signaling at central and peripheral targets. 5-HT4 receptor activation plays a role in learning and memory, feeding control and stress response. 5-HT4 receptors are encoded by a complex gene which in humans, generates ten different splice variants1, 2.5-HT4 receptor is a member of the seven transmembrane spanning G-protein-coupled receptors (GPCRs) and couples via Gs to increase cAMP production. GPCRs share a similar topology, with seven transmembrane helices (TMHs) connected by three extracellular loops (ECLs), and three intracellular loops (ICLs), the N-terminus is on the extracellular side of the membrane, and the C-terminus is cytoplasmic3.Within the central nervous system, these receptors are expressed in limbic structures including: hypothalamus, nucleus accumbens, prefrontal cortex, amygdala and hippocampus, where they contribute to emotional and cognitive functions4.Low levels of 5-HT4 receptors are associated with numerous neuropsychiatric disorders including Alzheimer's disease, Huntington's disease, feeding disorders and stress-related disorders such as anxiety and depression5.

Alternative names: 5HT4 Receptor (HTR4), 5-Hydroxytryptamine receptor 4, Serotonin receptor 4

Gene ID: 25324

NCBI Accession: NM_000870

UniProt: Q62758

Pathways: JAK-STAT Signaling, cAMP Metabolic Process, S100 Proteins