GLUT1 antibody (Extracellular)

Catalog No. ABIN7043692

Product Details anti-GLUT1 Antibody

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Binding Specificity: AA 41-55, Extracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLUT1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purpose: A Rabbit Polyclonal Antibody to Glucose Transporter 1

Specificity: 1st extracellular loop

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - 14 out of 15 amino acid residues identical

Characteristics: Anti-GLUT1 (extracellular) Antibody (ABIN7043692, ABIN7044497 and ABIN7044498) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GLUT1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EEFYNQTWNHRYGES, corresponding to amino acid residues 41-55 of rat Glucose transporter 1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7236994)

Blocking Peptide: (ABIN7236994)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GLUT1

Target: GLUT1 (SLC2A1) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 1 (SLC2A1))

Alternative Name: SLC2A1 (SLC2A1 Products)

Background: Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1,Glucose transporter 1 (GLUT1) belongs to the major facilitator superfamily (MFS). It is encoded by SLC2A1, and mediates basal-level cellular uptake of glucose into many tissues. GLUT1 contains 12 membrane-spanning domains with both the amino and carboxyl termini oriented intracellularly. In addition, a single extracellular N-linked glycosylation site is present1.GLUT1 is widely expressed, but it is most abundant in fibroblasts, erythrocytes, and endothelial cells with low levels of expression in muscle, liver, and adipose tissue2.Inactivating mutations of GLUT1, resulting in compromised transport activities for glucose, are associated with diseases as a result of lack of energy supply to the brain3. GLUT1 deficiency syndrome (also known as De Vivo syndrome) is characterized by a spectrum of symptoms including early-onset seizures, microcephaly and retarded development4. In addition, elevated expression levels of GLUT1 have been observed in several cancer types, identifying GLUT1 as an important prognostic indicator for tumorigenesis5.

Alternative names: GLUT1, Glucose transporter 1, Facilitated glucose transporter member 1, SLC2A1

Gene ID: 24778

NCBI Accession: NM_006516

UniProt: P11167

Pathways: Sensory Perception of Sound, Dicarboxylic Acid Transport, Warburg Effect