STOML1 antibody (Extracellular, N-Term)

Catalog No. ABIN7886509

The Rabbit Polyclonal anti-STOML1 antibody (ABIN7886509) specifically detects STOML1 in WB. The antibody is reactive with Mouse samples.

Quick Overview for STOML1 antibody (Extracellular, N-Term) (ABIN7886509)

Target: STOML1 (Stomatin (EPB72)-Like 1 (STOML1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This STOML1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-STOML1 Antibody

Binding Specificity: AA 4-17, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to Stomatin-Like Protein 1

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-Stomatin-like Protein 1 (extracellular) Antibody (ABIN7043778, ABIN7044516 and ABIN7044517) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize STOML1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RSGYRALPLGDFDR, corresponding to amino acid residues 4-17 of mouse STOML1

Isotype: IgG

Application Details

Application Notes:

WB: 1:500-1:800

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237284)

Blocking Peptide: (ABIN7237284)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for STOML1

Target: STOML1 (Stomatin (EPB72)-Like 1 (STOML1))

Alternative Name: STOML1

Background:

Synonyms: STOML1, SLP-1, STORP, Slipin-1, hUNC-24

Description: The Stomatin-like protein family contains five members and are characterized by the presence of a structurally conserved core called the stomatin-domain. This stomatin-domain is further related to a domain found in members of the prohibitin, flotillin and HflK/HflC protein families and has accordingly also been called the SPFH domain.All mammalian stomatin proteins are integral membrane protein with a single, relatively short, hydrophobic membrane insertion domain, followed by the core stomatin-domain. A single conserved proline residue (known to be a potent helix breaker) in the hydrophobic region is required for the formation of the protein's hairpin structure. The N-terminal domain is extracellular. Stomatin-like protein 1 (STOML1) has a typical stomatin-domain at the N-terminus and an additional sterol carrier protein-2 (SCP-2) domain at the C-terminus. STOML-1 is targeted to the late endosomal compartment where it interacts with stomatin. STOML-1 is predominantly expressed in the brain, in a subpopulation of sensory neurons in the dorsal root ganglia. Much lower levels are detected in other tissues1.Following anoxia, STOML-1 expression is up-regulated by the oxidative response factor SKN-1/Nrf. SKN-1/Nrf is required for both rapid mitochondrial refusion and rapid behavioral recovery during reoxygenation suggesting that STOML-1 helps facilitate mitochondrial dynamics following anoxia2.STOML-1 also modulates the proton-sensitive members of the acid-sensing ion channel (ASIC) family. STOML-1 strongly inhibits ASIC1a and accelerates the inactivation time constant of ASIC3, but has no effect on the splice variant ASIC1b3.

Gene ID: 69106

NCBI Accession: NM_004809

UniProt: Q8CI66