SYN1 antibody (Intracellular)

Catalog No. ABIN7043786

The Rabbit Polyclonal anti-SYN1 antibody has been validated for WB, IHC and IF. It is suitable to detect SYN1 in samples from Rat.

Quick Overview for SYN1 antibody (Intracellular) (ABIN7043786)

Target: SYN1 (Synapsin I (SYN1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-SYN1 Antibody

Binding Specificity: AA 234-245, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Synapsin I

Specificity: Intracellular, region C

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse,human - identical

Characteristics: Anti-Synapsin I (SYN1) Antibody is directed against an epitope of rat Synapsin I. Anti-Synapsin I (SYN1) Antibody (ABIN7043786, ABIN7044658 and ABIN7044659) can be used in western blot and immunohistochemistry applications. It has been designed to recognize Synapsin I from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RLHKKLGTEEFP(G), corresponding to amino acid residues 234 - 245 of rat Synapsin-1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:400

Comment:

Negative Control: (ABIN7237307)

Blocking Peptide: (ABIN7237307)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: SYN1

Background: Synapsin-1,Synapsins are a family of synaptic vesicle associated phosphoproteins consisting of three members: SYN1, SYN2, and SYN3, with several alternative splicing leading to 10 different isoforms. They are involved in synaptic transmission and plasticity, several stages of neurodevelopment, such as axon outgrowth and synapse formation. Synapsins are localized in presynaptic nerve terminals of central neurons1.Synapsin I is a peripheral membrane protein of synaptic vesicles that is phosphorylated at least at three different sites by various protein kinases. All synapsins have a single phosphorylation site in the short N-terminal domain A (site 1) that regulates neurite elongation and synaptic vesicle mobilization2.Synapsin I exhibits an asymmetric structure and exists in two forms, designated synapsin Ia and synapsin Ib. The primary structure of synapsin I is highly conserved, 95 % of the protein's residues are invariant and contain two major structural domains. The protein is divided into a hydrophobic, collagenase resistant head region and a proline rich, collagenase sensitive tail3,4. Synapsin I has been suggested to play an important role in the regulation of neurotransmitter release and the organization of cytoskeletal organization including actin filaments, microtubules and spectrin in the presynaptic terminal5.In vitro studies show that the dephospho-form of synapsin I binds synaptic vesicles, binds and bundles F-actin, and promotes G actin polymerization5.

Alternative names: Synapsin I (SYN1), Synapsin-1

Gene ID: 24949

NCBI Accession: NM_006950

UniProt: P09951