Synaptophysin antibody (Intravesicular Loop)

Catalog No. ABIN7886617

The Guinea Pig Polyclonal anti-Synaptophysin antibody (ABIN7886617) specifically detects Synaptophysin in WB and IHC. The antibody is reactive with Rat samples.

Quick Overview for Synaptophysin antibody (Intravesicular Loop) (ABIN7886617)

Target: Synaptophysin (SYP)

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Synaptophysin Antibody

Binding Specificity: AA 178-190, Intravesicular Loop

Purpose: A Guinea Pig Polyclonal Antibody to Synaptophysin.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rat,mouse,human - 12 out of 13 amino acid residues identical

Characteristics: A Guinea Pig Polyclonal Antibody to Synaptophysin.

Purification: Affinity purified on immobilized antigen.

Immunogen: CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7237323)

Blocking Peptide: (ABIN7237323)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: SYP

Background:

Synonyms: Major synaptic vesicle protein p38, SYP

Description: Synaptophysin is the major integral membrane protein of small synaptic vesicles1-3. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini4. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin5. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined6.Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons6.Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles.Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits7,8. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal6.

Gene ID: 24804

NCBI Accession: NM_003179

UniProt: P07825

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity