Recombinant IL-2 antibody

Catalog No. ABIN7072227

This anti-IL-2 antibody is a Rabbit Chimeric antibody detecting IL-2 in WB, ELISA, FACS, IF, Crys and LA. Suitable for Human.

Quick Overview for Recombinant IL-2 antibody (ABIN7072227)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Crystallization (Crys), Latex Agglutination (LA)

Clone: LNKB-2

Product Details anti-IL-2 Antibody

Purpose: Anti-IL-2 [LNKB-2], Rabbit IgG, kappa

Specificity: This antibody binds human IL-2 at the 59-72 site of the IL-2 sequence.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Isotype: IgG kappa

Application Details

Application Notes: This antibody was quantified by ELISA and used for latex-agglutination analysis of human IL-2. It was measured to bind human IL-2 with high affinity (Ka ~ 3 x 10⁸ M^-1) (Lunev et al., 1990, PMID: 1718463). This antibody has been used successfully for WB and FACS (Stratieva-Taneeva et al., 1993, PMID: 8359822). The crystal structure characterization of the Fab version of this antibody was performed (Fokin et al., 2000, PMID: 11040993). Further crystallization of the Fab version of this antibody with an antigenic peptide revealed that the antibody-antigen complexation involves a significant rearrangement of the epitope-containing region of the IL-2 with retention of the α-helical character of the epitope fragment (Afonin et al., 2001, PMID: 11468348).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background: Interleukin 2, LNKB2

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation