Recombinant EGFR antibody

Catalog No. ABIN7072324

This Rabbit Chimeric antibody specifically detects EGFR in WB, IHC, ELISA, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for Recombinant EGFR antibody (ABIN7072324)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: S3 12D

Product Details anti-EGFR Antibody

Purpose: Anti-EGFR [S3 12D], Rabbit IgG, kappa

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: scFv antibody fragment

Purification: Protein A affinity purified

Immunogen: Human EGFR.

Isotype: IgG kappa

Application Details

Application Notes: The antibody binds specifically to EGFR, a cell-surface receptor which is part of the ErbB family of receptors. The EGFR is a receptor tyrosine kinase, which, upon activation by its ligands, such as epidermal growth factor and transforming growth factor alpha, triggers signal transduction pathways that increase cell migration, adhesion and proliferation. Mutations that lead to the overexpression of EGFR are associated with the development of cancer. The S3 12D antibody is prepared from isolation of phage antibodies of the spleen library.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv antibody fragment format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background: Epidermal growth factor receptor, ERBB, ERBB1, HER1, mENA, PIG61, NISBD2

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins