Recombinant GM2 Ganglioside antibody

Catalog No. ABIN7072396

This Rabbit Chimeric antibody specifically detects GM2 Ganglioside in ELISA, IHC, Dep and TLC Overlay. It exhibits reactivity toward Human.

Quick Overview for Recombinant GM2 Ganglioside antibody (ABIN7072396)

Target: GM2 Ganglioside

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Depletion (Dep), Thin Layer Chromatography Overlay (TLC Overlay)

Clone: L55-81

Product Details

Purpose: Anti-GM2 ganglioside [L55-81], Rabbit IgG, kappa

Specificity: L55-81 binds specifically to GM2 ganglioside, this antibody shows no reactivity towards GM1, GM3, GD2 or normal tissues- the L55-81 epitope contains both a GalNAc and sialic acid bound to lactose. Ganglioside GM2 (GalNAcβ1-4(NeuNAcα2-3)Galβ1-4Glcβ-1ceramide) is a glycosphingolipid present on the cell surface and a major tumor-differentiation antigen in human melanoma and hepatoma.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgM

Purification: Protein A affinity purified

Immunogen: L55-81 was prepared by isolating EBV-immortalized peripheral blood B lymphocytes from a human donor with melanoma.

Isotype: IgG kappa

Application Details

Application Notes: L55-81 reacts positively to melanoma cell lines with high GM2 expression (M14, M24), and shows no binding to normal cell types or cell lines M15 or M25 which have low GM2 expression and high GM3 expression. This specificity can be confirmed by ELISA and TLC immunostaining by testing a variety of gangliosides and neutral glycolipids. L55-81 is able to lyse cells from the M14 melanoma cell line (which has high GM2 expression) in the presence of human or rabbit complement, but not in the absence of complement - this was shown by a [51Cr] release assay. Biotinylated L55-81 has been used for IHC of acetone-fixed sections of tumor tissue in an avidin-biotin complex immunoperoxidase system. Statistically significant positive staining (determined by Fisher's exact test) was displayed with melanoma, colon carcinoma and ovary carcinoma tumor tissues. Staining has also be observed with breast carcinoma, prostate carcinoma and kidney carcinoma.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: GM2 Ganglioside