Recombinant GM2 Ganglioside antibody

Catalog No. ABIN7543164

The Human Monoclonal anti-GM2 Ganglioside antibody is suitable to detect GM2 Ganglioside in samples from Human. It has been validated for ELISA, IF, FACS and ICC.

Quick Overview for Recombinant GM2 Ganglioside antibody (ABIN7543164)

Target: GM2 Ganglioside

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Human

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: KM966

Product Details

Purpose: Anti-GM2 ganglioside [KM966], Human IgG1, kappa

Specificity: This antibody recognizes and binds ganglioside GM2.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This chimeric antibody was generated by cloning the variable heavy and light chain sequences of murine parent antibody KM696 (IgM) and expressing it as a mouse/human IgG1. The parent antibody was raised against human GM2.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody shows antitumor activity both in vitro and in vivo. Chimeric KM966 markedly suppressed the establishment of f human tumor xenografts in nude mice. Indirect immunofluorescence staining of tumor cell lines with the chimeric KM966 revealed that the antigen was expressed in substantial amounts on pulmonary tumor cells and leukemia cells as well as neuroectodermal origin tumor cells. This antibody also reacts with GM2 in an ELISA and also can be used for the identification of tumor cells expressing GM2 via flow cytometry (PMID: 8137257). The effect of chimeric antibody KM966 on GM2 expressing human solid tumors in vivo was done in PMID: 10417777. This antibody was also used in the immunocytochemical staining of cultured skin fibroblast of patients with GM2-gangliosidosis (PMID: 28417072). Humanized versions of this antibody KM8966, KM8967, KM8969 and KM8970 are available on request.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: GM2 Ganglioside

Background: GM2