Recombinant CD25 antibody

Catalog No. ABIN7072444

This Mouse Monoclonal antibody specifically detects CD25 in FACS, ELISA and BR. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD25 antibody (ABIN7072444)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Clone: AHT107

Product Details anti-CD25 Antibody

Purpose: Anti-IL2R [AHT107], Mouse IgG1, kappa

Specificity: AHT107 binds to a different epitope than IL-2. Suggested that determinant recognised by AHT107 is involved in formation of the high-affinity binding site of the p55 + p75 heterodimeric complex of the IL-2R in a way which does not disturb the interaction of the p55 Molecule and IL-2, and that the high-affinity class of the IL-2R is responsible for mediating the growth signal at physiological concentrations of IL-2.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: BALB/c mice were immunized with human T lymphoblasts and spleen cells of the immunized animals were fused with X63-Ag8.653 mouse myeloma cells. The supernatants of the resultant hybridomas were screened by a proliferation inhibition assay (inhibition of the human T lymphoblast response to IL-2). Two of those (the clones AHT54 and AHT107) that produced antibodies which inhibited IL-2 dependent proliferation of human T lymphoblasts were selected, cloned and recloned.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody has been used to create a chimeric antibody with a murine variable region and human constant region through the use of recombinant DNA techniques. This gave antigen specificity of the mouse, and immunogenicity, serum half-life, and effector functions of human antibodies. The chimeric antibody has been found to have a greater anti-proliferative effect on T lymphocytes (Rose 1991). AHT107 has been used in ELISAs for the detection of soluble human IL-2R in response to different forms of helminthic and protozoal infections (O. Josimovic et al. 1988).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL2R

Background: CD25, Interleukin 2 receptor, IL-2R, IL-2 receptor, IL2 receptor, Tac antigen, p55, AHT-107, AHT107

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation