Recombinant CD25 antibody

Catalog No. ABIN7072445

The Rabbit Chimeric anti-CD25 antibody has been validated for FACS, ELISA and BR. It is suitable to detect CD25 in samples from Human.

Quick Overview for Recombinant CD25 antibody (ABIN7072445)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Clone: AHT107

Product Details anti-CD25 Antibody

Purpose: Anti-IL2R [AHT107], Rabbit IgG, kappa

Specificity: AHT107 binds to a different epitope than IL-2. Suggested that determinant recognised by AHT107 is involved in formation of the high-affinity binding site of the p55 + p75 heterodimeric complex of the IL-2R in a way which does not disturb the interaction of the p55 Molecule and IL-2, and that the high-affinity class of the IL-2R is responsible for mediating the growth signal at physiological concentrations of IL-2.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: BALB/c mice were immunized with human T lymphoblasts and spleen cells of the immunized animals were fused with X63-Ag8.653 mouse myeloma cells. The supernatants of the resultant hybridomas were screened by a proliferation inhibition assay (inhibition of the human T lymphoblast response to IL-2). Two of those (the clones AHT54 and AHT107) that produced antibodies which inhibited IL-2 dependent proliferation of human T lymphoblasts were selected, cloned and recloned.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used to create a chimeric antibody with a murine variable region and human constant region through the use of recombinant DNA techniques. This gave antigen specificity of the mouse, and immunogenicity, serum half-life, and effector functions of human antibodies. The chimeric antibody has been found to have a greater anti-proliferative effect on T lymphocytes (Rose 1991). AHT107 has been used in ELISAs for the detection of soluble human IL-2R in response to different forms of helminthic and protozoal infections (O. Josimovic et al. 1988).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: IL2R

Background: CD25, Interleukin 2 receptor, IL-2R, IL-2 receptor, IL2 receptor, Tac antigen, p55, AHT-107, AHT107

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation