Recombinant CD80 antibody

Catalog No. ABIN7072474

This Rat Monoclonal antibody specifically detects CD80 in FACS, WB, IHC, Func and BP. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant CD80 antibody (ABIN7072474)

Target: CD80

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD80 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Functional Studies (Func), Blocking Peptide (BP)

Clone: RM80

Product Details anti-CD80 Antibody

Purpose: Anti-CD80 [RM80], Rat IgG2a, kappa

Specificity: This antibody is specific for murine CD80, a ~55 kDa member of the Ig superfamily. CD80 is expressed by macrophages, dendritic cells, activated B cells, and activated T cells. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to the activation of T and B cells, respectively.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody has been used in multiple FACS experiments for various immunological applications, such as to highlight the robustness of the T cell priming process by activated Nlrc5-deficient dendritic cells (Rota et al, 2016), to demonstrate that allogeneic IgG combined with dendritic cell stimuli could induce anti-tumor T cell immunity (Carmi et al, 2015), to confirm the role of aquaporin-9-expressing neutrophils for the development of contact hypersensitivity (Moniaga et al, 2015), and to support the therapeutic potential of targeting B-cell Syk signaling in chronic graft-versus-host disease (Flynn et al, 2015). This antibody has also been used in Western Blot to study the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al, 2015), as well as in in vitro blocking experiments to confirm the role of CD28 signals for IL-2-mediated survival and proliferation of both CD27(+) and CD27(-) γδ T cell subsets (Ribot et al, 2012). Furthermore, this antibody has been used, together with the anti-CD86 antibody, in numerous in vivo studies, such as in a NZB/W F1 mouse lupus model to clarify the role of CD80 and CD86 in murine Ab-mediated autoimmunity (Nakajima et al, 1995), in graft bearing recipient BALB/c mice to evaluate the roles of CD80 and CD86 costimulatory molecules in corneal allograft rejection (Kagaya et al, 2002), and in transgenic BALB/c mice to demonstrate the critical contribution of CD80 and CD86 in anterior chamber-associated immune deviation (Tsukahara et al, 2005).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background: B7-1, Activation B7-1 antigen, B7, B7 protein, B7-1 protein, B7.1, Cd28l, CD28LG, CD28LG1, LAB7, Ly-53, Ly53, MIC17, T-cell co-stimulatory protein B7-1, T-lymphocyte activation antigen CD80, TS/A-1

UniProt: Q00609

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints