Recombinant CD25 antibody

Catalog No. ABIN7072513

This Rabbit Chimeric antibody specifically detects CD25 in FACS, WB, ELISA, IF, Func and Dep. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant CD25 antibody (ABIN7072513)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD25 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunofluorescence (IF), Functional Studies (Func), Depletion (Dep)

Clone: PC-61-5-3

Product Details anti-CD25 Antibody

Purpose: Anti-CD25 [PC-61.5.3], Rabbit IgG, lambda

Specificity: This antibody is specific for murine CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising rats with the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells with P3X63Ag8.653 myeloma cells.

Isotype: IgG lambda

Application Details

Application Notes: This antibody has been used in numerous FACS analyses, for instance, to demonstrate that glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N-glycosylation (Araujo et al, 2017), to suggest that IL-2C could be a potential therapeutic method to alleviate excessive inflammation and maintain blood vessel stability after traumatic brain injury (Gao et al, 2017), and to evaluate how alternative splicing of MALT1 controls signalling and activation of CD4(+) T cells (Meininger e al, 2016). This antibody has also been used in immunofluorescence as part of the DNA Aβ42 immunization studies in mice (Lambracht-Washington et al, 2015), in ELISA assays to investigate the dynamics of T cell receptor (TCR)-dependent signaling networks (Brockmeyer et al, 2011), and in Western Blot to determine the effects of increased p300 expression on glucocorticoid receptor (GR)-T-cell-receptor (TCR) crosstalk between thymocytes (Yu et at, 2002). In addition, this antibody has been used in various in vivo functional studies, for instance, to suggest that a combination of local anti-CTLA-4 antibody production with systemic Treg depletion could enhance antitumor immune responses (Tuve et al, 2007), to provide a new insight into infection-associated tumorigenesis and illustrate the importance of antibiotic therapy to treat tumors with bacterial infiltration (Huang et al, 2007), and to demonstrate that CD4+CD25+ T cells may downregulate the development of glomerulonephritis during the preactive phase in B/WF1 mice (Hayashi et al, 2005).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background: IL2-RA, IL-2 receptor subunit alpha,L-2R alpha chain, IL-2R subunit alpha, Interleukin-2 receptor subunit alpha, p55, p55 chain, PC61, PC6153

UniProt: P01590

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation