Recombinant CD4 antibody

Catalog No. ABIN7072528

This Rabbit Chimeric antibody specifically detects CD4 in FACS, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD4 antibody (ABIN7072528)

Target: CD4

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Neutralization (Neut)

Clone: DB81

Product Details anti-CD4 Antibody

Purpose: Anti-CD4 [DB81], Rabbit IgG, kappa

Specificity: This antibody is specific for human CD4, and recognises an epitope in the vicinity of a 13-amino acid loop in domain 2 of CD4. It cross-reacts with chimpanzee CD4, but not baboon or macaque CD4.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising BALB/c mice with human CD4 complexed with HIV-1 Env (gp120).

Isotype: IgG kappa

Application Details

Application Notes: This antibody preferentially binds to a complex-enhanced epitope on the D2 domain of human CD4. Plasmon resonance analysis has shown that the binding affinity of this antibody to the CD4-gp120 complex is almost one order of magnitude higher than its binding to CD4 alone (Burastero et al, 2011), while ELISA analysis has confirmed that the epitope recognised by this antibody does not involve gp120 (Burastero et al, 2011). This antibody, through a post-binding mechanism, inhibits the cell fusion and replication of a range of HIV-1 strains (Burastero et al, 2011). It does not exert inhibitory effects on T-cell activation in vitro, a useful property for clinical use.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: Lu/T4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events