Recombinant RAGE antibody

Catalog No. ABIN7072541

The Rabbit Chimeric anti-RAGE antibody has been validated for WB, IHC, ELISA, FACS, IF and SPR. It is suitable to detect RAGE in samples from Human.

Quick Overview for Recombinant RAGE antibody (ABIN7072541)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This RAGE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Surface Plasmon Resonance (SPR)

Clone: 2A11

Product Details anti-RAGE Antibody

Purpose: Anti-RAGE [2A11], Rabbit IgG, kappa

Specificity: 2A11 is specific for the V-domain of human advanced glycosylation end product-specific receptor. It recognises both the glycosylated and non-glycosylated forms.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: Human RAGE protein.

Isotype: IgG kappa

Application Details

Application Notes: ELISA confirmed the specificity of 2A11 for the V-domain of RAGE, with a binding affinity (Kd) of 7.6 nM. ELISA was also used to confirm that the binding of 2A11 to RAGE is glycosylation independent. In SPR experiments the Kd of 2A11 binding to RAGE was determined as 0.38 nM. Flow cytometry and immunochemistry were used to visualise the binding of 2A11 to HEK-RAGE cells.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: RAGE

Background: AGER, advanced glycosylation end product-specific receptor, receptor for advanced glycosylation end products

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins