Recombinant PD-1 antibody (Extracellular Domain)

Catalog No. ABIN7072592

The Rabbit Chimeric anti-PD-1 antibody is suitable to detect PD-1 in samples from Mouse. It has been validated for FACS, IHC, ELISA, IP, Neut and Dep.

Quick Overview for Recombinant PD-1 antibody (Extracellular Domain) (ABIN7072592)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Neutralization (Neut), Depletion (Dep)

Clone: J43

Product Details anti-PD-1 Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-PD-1 [J43], Rabbit IgG, lambda

Specificity:

This antibody is specific for murine PD-1.

Application Notes original Clone: The specificity of this antibody has been confirmed in ELISA analysis, using PD-1 extracellular domain fusion proteins (Agata et al, 1996). Additionally, in flow cytometric analysis, this antibody reacts with PD-1 cDNA-transfected BHK and CHO cells, but not with parental BHK and CHO cells, as well as reacting with lymphocytes from PD-1 cDNA transgenic mice (Agata et al, 1996). This antibody has been used to immunoprecipitate PD-1 from lysates of PD-1 cDNA-transfected BHK and CHO cells (Agata et al, 1996), in flow cytometric quantification of CD4+PD-1+ T cells in murine spleens (Kasagi et al, 2010), and in immunohistochemical analysis of acetone-fixed murine spinal cord and brain tissue sections (Salama et al, 2003). This antibody displays diverse effects in different mouse models of disease. When administered to NZB/W F1 mice, a model of lupus-like nephritis, this antibody has been shown to delay the onset of nephritis and prolong survival, through the depletion of PD-1+ T cells (Kasagi et al, 2010). Antibody-treated NZB/W F1 mice displayed decreased numbers of PD-1+ T cells, and this antibody was confirmed to trigger complement-dependent cytotoxicity in PD-1+ T cells in vitro (Kasagi et al, 2010). Conversely, administration to experimental allergic encephalitis (EAE) and NOD diabetes mice exacerbated disease, through its neutralising activity (Salama et al, 2003, Ansari et al, 2003), this antibody has been shown in vitro to inhibit binding of both PD-L1-Ig and PD-L2-Ig to PD-1 transfected BHK cells (Ansari et al, 2003).

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Armenian hamsters with B12 cells, a PD-1 cDNA transfectant of BHK cells.

Isotype: IgG, lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Hamster, Armenian

OriginalFormat: IgG

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PD-1

Background: CD279, PD1, PD 1, Programmed cell death protein 1, Programmed cell death 1, pdcd1, Protein PD-1, mPD-1

UniProt: Q02242

Pathways: Cancer Immune Checkpoints