Recombinant CSF1R antibody

Catalog No. ABIN7072608

This anti-CSF1R antibody is a Rabbit Chimeric antibody detecting CSF1R in WB, IHC, FACS and BP. Suitable for Mouse.

Quick Overview for Recombinant CSF1R antibody (ABIN7072608)

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CSF1R antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Blocking Peptide (BP)

Clone: AFS98

Product Details anti-CSF1R Antibody

Purpose: Anti-CD115/M-CSFR [AFS98], Rabbit IgG, kappa

Specificity: This antibody recognises mouse M-CSFR (CD115, CSF-1R) which is a membrane protein that influences tissue macrophage and osteoclast differentiation and proliferation.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised against CD115.

Isotype: IgG kappa

Application Details

Application Notes: AFS98 has been widely used in various flow cytometric analyses to label CD115 antigen. For instance, one group used it to detect monocytes in a mouse blood sample (Buschor et al., 2019). Similarly, Huo and colleagues (2017) utilised this antibody to identify monocytes in mouse blood in order to assess whether myelopoiesis was not a cause of the increase in the monocytes number. This antibody has been also successfully used to deplete macrophages (Ho et al., 2016). Finally, it was also utilised in the immunofluorescence staining of tissue sections prepared from 4 % paraformaldehyde-fixed and decalcified bones (Dong et al., 2016).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CSF1R

Target: CSF1R (Colony Stimulating Factor 1 Receptor (CSF1R))

Alternative Name: CD115/M-CSFR

Background: CSF-1R, Macrophage colony-stimulating factor 1 receptor, Csf1r, CD115, CSF-1 receptor, EC 2.7.10.1, CSF-1-R, M-CSF-R, Proto-oncogene c-Fms

UniProt: P09581

Pathways: RTK Signaling, Inositol Metabolic Process, Cell-Cell Junction Organization