Recombinant CD15 antibody

Catalog No. ABIN7072624

This Rabbit Chimeric antibody specifically detects CD15 in FACS, IHC, IF and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD15 antibody (ABIN7072624)

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD15 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: BRA-4F1

Product Details anti-CD15 Antibody

Purpose: Anti-CD15 [BRA-4F1], Rabbit IgG, kappa

Specificity: This antibody is specific for CD15 (Appelmelk. et al, 1996, pmid:8675304). It also shows crossreactivity with the CD15 antigen from other species.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgM

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunzing mice with human CD15.

Isotype: IgG kappa

Application Details

Application Notes: This antibody has been used in flow cytometry (Appelmelk. et al, 1996). The antibody has been shown to be usefull in immunohistochemistry (Appelmelk. et al, 1996). The antibody is utilized in immunopercipitation (Akin. et al, 2000). Immunofluorescence is a proven application of this antibody (Vilella. et al, 2003). CD15 is expressed in Reed-Sternberg cells, which are a sign of Hodgkin's lymphoma, and thus the antibody can be used in immunohistochemistry to detect this kind of cancer (Trillo-Tinoco. et al, 2019). Finally, BRA-4F1 can be used in fertility research as the CD15 antigen is a molecule that is nessecary for a sperm cell to recognize and fertilize an egg cell.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD15

Target: CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))

Alternative Name: CD15

Background: SLeX, Lewis X antigen, Leu-M1, Sialyl-Lewis X, SSEA-1, sialyl LeX