Recombinant CCR7 antibody

Catalog No. ABIN7072673

The Rat Monoclonal anti-CCR7 antibody has been validated for FACS, IP and Func. It is suitable to detect CCR7 in samples from Mouse.

Quick Overview for Recombinant CCR7 antibody (ABIN7072673)

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CCR7 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)

Clone: 4B12

Product Details anti-CCR7 Antibody

Purpose: Anti-CCR7 [4B12], Rat IgG2a, kappa

Specificity: This antibody is specific to the mouse CCR7 protein, which was confirmed by immunoprecipitation (Ritter et al, 2004).

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing rats with mouse CCR7 antigen.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody was used for flow cytometry and the staining quality can be improved by incubation at 37 °C (Britschgi et al, 2008). This antibody is widely used to monitor the activation of dendritic cells in mice (Ohl et al, 2004, Ritter et al, 2004, Zhao et al, 2019). It was also utilized in immunoprecipitation of the CCR7 receptor protein (Ritter et al, 2004). The antibody does not interfere with ligands binding to the CCR7 receptor, which was confirmed by a transmigration assay (Ritter et al, 2004). It does not interfere with T-cell migration (Ritter et al, 2004). The antibody also is not agonistic as it does not activate the receptor (Ritter et al, 2004).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CCR7

Target: CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))

Alternative Name: CCR7

Background: EBI1, EBV-induced G-protein coupled receptor 1, Epstein-Barr virus-induced G-protein coupled receptor 1, MIP-3 beta receptor, CD197, C-C CKR-7, CC-CKR-7, C-C chemokine receptor type 7

UniProt: P47774

Pathways: Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process