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Recombinant Cytokeratin 7 antibody

KRT7 Reactivity: Human WB, IHC, IF, FACS Host: Rabbit Chimeric OV-TL 12-30 unconjugated Recombinant Antibody
Catalog No. ABIN7072675
  • Target See all Cytokeratin 7 (KRT7) Antibodies
    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 208
    • 72
    • 62
    • 7
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 122
    • 94
    • 1
    Rabbit
    Clonality
    • 122
    • 95
    Chimeric
    Conjugate
    • 93
    • 19
    • 16
    • 6
    • 6
    • 6
    • 6
    • 6
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    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
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    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Cytokeratin 7 antibody is un-conjugated
    Application
    • 128
    • 86
    • 86
    • 82
    • 39
    • 30
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    • 26
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    • 22
    • 19
    • 16
    • 5
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    • 1
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    Anti-Cytokeratin 7 [OV-TL 12/30], Rabbit IgG, kappa
    Specificity
    This antibody recognizes cytokeratin-7. Cytokeratins are a subfamily of intermediate filament proteins but this antibody does not recognize other intermediate filament proteins. The antibody reacts with proteins that are found in most ductal, glandular, transitional, and biliary duct epithelial cells.
    Characteristics

    Original Species of Ab: Mouse

    Original Format of Ab: IgG1

    Purification
    Protein A affinity purified
    Immunogen
    This antibody was originally raised by immunizing BALB/c mice with ovarian carcinoma cell line OTN11.
    Clone
    OV-TL 12-30
    Isotype
    IgG kappa
    Top Product
    Discover our top product KRT7 Primary Antibody
  • Application Notes
    Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometry. Cytokeratin 7 (CK7) labeling can help distinguish between lung, breast carcinomas, and urothelial carcinomas that typically stain positive, and colon and prostate carcinomas that typically lack CK7 expression (Jerome et al., 2004, Logani et al., 2003, Murray et al., 2004, Roy et al., 2011). CK7 is a common marker of primary lung adenocarcinomas (almost all cases) with a lower specificity since it is also observed in other primary lung carcinomas and non-pulmonary carcinomas (Jerome et al., 2004). Anti-cytokeratin 7 has also been useful in the differential diagnosis of ovarian neoplasms (McCluggage et al., 2005). Furthermore, this antibody is a marker for distinguishing between the conjunctival and corneal surface epithelia which may be used in the diagnosis of limbal stem cell deficiency (LSCD) (Jirsova et al., 2011, pmid: 21693612). Finally, OV-TL 12/30 has proven very effective and popular in immunohistochemistry, for instance, one group used it to label cytokeratin-7 in human term placental samples (König et al., 2014, pmid: 24326460).
    Comment

    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
    Alternative Name
    Cytokeratin 7 (KRT7 Products)
    Background
    CK-7, Keratin, type II cytoskeletal 7, Cytokeratin-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7, CK7, CK 7
    UniProt
    P08729
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