UCHL1 antibody (AA 35-140) (HRP)

Catalog No. ABIN704405

The Rabbit Polyclonal anti-UCHL1 antibody is suitable to detect UCHL1 in samples from Human, Rat, Mouse, Cow, Goat and Chicken. It has been validated for WB, ELISA, IHC (p) and IHC (fro).

Quick Overview for UCHL1 antibody (AA 35-140) (HRP) (ABIN704405)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Rat, Mouse, Cow, Goat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-UCHL1 Antibody (HRP)

Binding Specificity: AA 35-140

Cross-Reactivity: Chicken, Cow, Goat, Human, Mouse, Rat

Predicted Reactivity: Pig,Horse,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PGP9.5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1/PGP9.5

Background:

Synonyms: NDGOA, PARK5, PGP95, PGP9.5, Uch-L1, HEL-117, PGP 9.5, Ubiquitin carboxyl-terminal hydrolase isozyme L1, Neuron cytoplasmic protein 9.5, Ubiquitin thioesterase L1, UCHL1

Background: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

Gene ID: 7345

UniProt: P09936

Pathways: Feeding Behaviour