CD45 antibody (mFluor™450)

Catalog No. ABIN7076605

The Mouse Monoclonal anti-CD45 antibody has been validated for FACS. It is suitable to detect CD45 in samples from Human.

Quick Overview for CD45 antibody (mFluor™450) (ABIN7076605)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45 antibody is conjugated to mFluor™450

Application: Flow Cytometry (FACS)

Grade: GMP Grade (Analyte)

Clone: F10-89-4

Product Details anti-CD45 Antibody (mFluor™450)

Purpose: CD45 mFluor™ 450 Antibody

Characteristics: The clone F10-89-4, a mouse monoclonal antibody, recognizes a hematopoietic cell surface antigen known as CD45 which is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and basophils in peripheral blood. CD45 has a role in signal transduction, modifying signals from other surface molecules. The CD45 antibody has been reported to react weakly with mature circulating erythrocytes and platelets. The antibody recognizes the human leukocyte common antigen which is found on cells from spleen, lymph nodes, 83 % bone marrow cells, and granulocytes. The antigen recognized by F10-89-4 is a glycoprotein with a molecular weight of 190 kDa to 215 kDa

Purification: Purified

Purity: >95 %

Immunogen: Human T lymphocytes

Isotype: IgG2a, kappa

Application Details

Application Notes: FC: 5 µL/test We recommend that every lab carries out an initial titration study before running your samples to ensure that the optimal concentration is selected for your application.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2, 0.09 % Sodium azide and 0.2 % (w/v) BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C, Conjugated antibodies should never be frozen.

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45

Gene ID: 5788

NCBI Accession: NM_002838

UniProt: P08575

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling