EZH2 antibody

Catalog No. ABIN7091061

This Mouse Monoclonal antibody specifically detects EZH2 in WB, FACS and IF (cc). It exhibits reactivity toward Human.

Quick Overview for EZH2 antibody (ABIN7091061)

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EZH2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: E3H5

Product Details anti-EZH2 Antibody

Purpose: EZH2 Monoclonal Antibody

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: Recombinant human EZH2.

Isotype: IgG1 kappa

Application Details

Application Notes: WB(WB(1:500-2000)), FCM(FCM(1 μg/Test)), IF(ICC)(IF(ICC)(1:50-200))

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for EZH2

Target: EZH2 (Enhancer of Zeste Homolog 2 (EZH2))

Alternative Name: EZH2

Background:

Synonyms: Enhancer of zeste homolog 2, Enx1h, MGC9169, Enhancer of zeste 2, ENX-1, ENX 1, ENX1, EZH 2, EZH2_HUMAN, Histone-lysine N-methyltransferase EZH2, KMT6A, Lysine N-methyltransferase 6, Enhancer of zeste homolog 2 (Drosophila), Enhancer of zeste, Drosophila, homolog 2, KMT 6,KMT6A, KMT6, WVS2.

Background: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.

Gene ID: 2146

UniProt: Q15910

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation