Recombinant IKZF1 antibody

Catalog No. ABIN7091238

This Rabbit Monoclonal antibody specifically detects IKZF1 in WB, IF, IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Recombinant IKZF1 antibody (ABIN7091238)

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IKZF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IKZF1 Antibody

Purpose: IKZF1 Recombinant Antibody

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IKZF1

Isotype: IgG

Application Details

Application Notes: WB(1:200-500), IHC-P(1:200-500), IHC-F(1:50-100), IF(1:50-100), ICC/IF(1:50-100)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M TBS ( pH 7.4), 1 % BSA, 0.02 % Proclin 300, and 50 % Glycerol

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for IKZF1

Target: IKZF1 (IKAROS Family Zinc Finger 1 (Ikaros) (IKZF1))

Alternative Name: IKZF1

Background:

Synonyms: IKAROS family zinc finger 1 (Ikaros), PRO0758, CLL associated antigen KW 6, DNA-binding protein Ikaros, hIk 1, hIk-1, IK1, Ikaros (zinc finger protein), IKAROS, Ikaros family zinc finger protein 1, IKZF1_HUMAN, LYF1, Lymphoid transcription factor LyF-1, zinc finger protein subfamily 1A 1, ZNFN1A1.

Background: This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014]

Gene ID: 10320

UniProt: Q13422

Pathways: Production of Molecular Mediator of Immune Response