ARF1 antibody

Catalog No. ABIN7111365

The Rabbit Polyclonal anti-ARF1 antibody (ABIN7111365) specifically detects ARF1 in WB, IHC, ELISA, IF and IP. The antibody is reactive with Human and Mouse samples.

Quick Overview for ARF1 antibody (ABIN7111365)

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-ARF1 Antibody

Purpose: ARF1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: peptide

Immunogen: ADP-ribosylation factor 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1

Background:

Synonyms: ADP-ribosylation factor 1|ARF1

Background: GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity, the function is linked to AMPA receptor(AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression(LTD).

Molecular Weight: 21 kDa

Gene ID: 375

UniProt: P84077

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process